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mouse e11 cdna library  (TaKaRa)


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    TaKaRa mouse e11 cdna library
    Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage <t>E11</t> mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.
    Mouse E11 Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+e11+cdna+library/Mate+%26+Plate+Library+-+Mouse+Embryo+11-day/pm36670152-257-2-7
    Average 93 stars, based on 12 article reviews
    mouse e11 cdna library - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3."

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.

    Journal: Scientific reports

    doi: 10.1038/s41598-023-27878-9

    Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage E11 mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.
    Figure Legend Snippet: Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage E11 mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.

    Techniques Used: Isolation, Binding Assay, Two Hybrid Screening, Construct

    Related Articles

    cDNA Library Assay:

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.
    Article Snippet: .. Therefore, as there were no appropriately staged, commercially available zebrafish Y2H libraries and we were most interested in identifying protein interactions that are conserved between species, we used mouse HMX3 as a bait protein and a mouse E11 cDNA library (Clontech Mate & Plate Mouse E11 Day Library) for the prey proteins. ..

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3
    Article Snippet: .. Therefore, as there were no appropriately staged, commercially available zebrafish Y2H libraries and we were most interested in identifying protein interactions that are conserved between species, we used mouse HMX3 as a bait protein and a mouse E11 cDNA library (Clontech Mate & Plate Mouse E11 Day Library) for the prey proteins. ..

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Clone Assay:

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Plasmid Preparation:

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Transformation Assay:

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.
    Article Snippet: .. We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain. ..



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    Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage <t>E11</t> mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.
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    Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage <t>E11</t> mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.
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    Image Search Results


    Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage E11 mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.

    Journal: Scientific reports

    Article Title: Analyses of binding partners and functional domains for the developmentally essential protein Hmx3a/HMX3.

    doi: 10.1038/s41598-023-27878-9

    Figure Lengend Snippet: Figure 1. Isolation of novel binding partners of HMX3 with yeast two-hybrid screening. Top: Schematic representation of bait and prey constructs used in screening. Full-length mouse HMX3 fused to the Gal4 DNA- binding domain (DBD) was used as bait. A library of stage E11 mouse cDNAs fused to the Gal4 activating domain (AD) was used as prey. 7.2 × 108 solid plate matings were performed, yielding 3200 positive colonies. Positive prey inserts were segregated and sequenced and reads were aligned to the mouse genome, identifying 851 unique genes. After removing non-coding genes and those for which only untranslated region (UTR) or intronic sequences were recovered, 539 protein-coding sequences remained. Zebrafish orthologs of the most functionally interesting genes were selected for further analysis with Co-IPs.

    Article Snippet: We used mouse E11 cDNA library (630478; Takara Bio) cloned into pGAD57 vector as prey, transformed into Y187 yeast strain.

    Techniques: Isolation, Binding Assay, Two Hybrid Screening, Construct